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Exposure to the environmentally relevant fungicide Maneb: studying toxicity in the[...]
3 Results and discussion
3.3 Neurotoxicity
2.1 Caenorhabditis elegans strain and maintenance
2.2 Materials and synthesis
2.3 Acute exposure
2.4 Survival assay
2.5 Measurement of Mn bioavailability
2.6 Protein determination via bicinchoninic acid (BCA) assay
2.7 Neurotransmitter quantification
2.8 Fluorescence microscopy of DAergic neurons
2.9 Carboxy-DCFH-DA-assay for RONS measurement
2.10 Adenine nucleotide quantification
2.11 GSH and GSSG quantification
2.12 Statistical analysis
3 Results and discussion
3.1 Acute toxicity
3.2 Bioavailability of Mn
3.3 Neurotoxicity
3.4 Reactive oxygen and nitrogen species (RONS) formation and adenine nucleotides
3.5 GSH and GSSG quantification
4 Conclusion
Funding
CRediT authorship contribution statement
Declaration of competing interest
Data availability
Acknowledgements
Appendix A Supplementary data
Reference
Aufsatz in einer Zeitschrift
Exposure to the environmentally relevant fungicide Maneb: studying toxicity in the soil nematode Caenorhabditis elegans
Entstehung
Wuppertal
2024
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