Kubens, Laura; Weishaupt, Ann-Kathrin; Michaelis, Vivien; Rohn, Isabelle; Mohr, Fabian; Bornhorst, Julia: Environment International / Exposure to the environmentally relevant fungicide Maneb: studying toxicity in the soil nematode Caenorhabditis elegans. In: Environment International. Wuppertal : Universitätsbibliothek, 2024
Content
- 2.1 Caenorhabditis elegans strain and maintenance
- 2.2 Materials and synthesis
- 2.3 Acute exposure
- 2.4 Survival assay
- 2.5 Measurement of Mn bioavailability
- 2.6 Protein determination via bicinchoninic acid (BCA) assay
- 2.7 Neurotransmitter quantification
- 2.8 Fluorescence microscopy of DAergic neurons
- 2.9 Carboxy-DCFH-DA-assay for RONS measurement
- 2.10 Adenine nucleotide quantification
- 2.11 GSH and GSSG quantification
- 2.12 Statistical analysis
- 3 Results and discussion
- 3.1 Acute toxicity
- 3.2 Bioavailability of Mn
- 3.3 Neurotoxicity
- 3.4 Reactive oxygen and nitrogen species (RONS) formation and adenine nucleotides
- 3.5 GSH and GSSG quantification
- 4 Conclusion
- Funding
- CRediT authorship contribution statement
- Declaration of competing interest
- Data availability
- Acknowledgements
- Appendix A Supplementary data
- Reference