Titelaufnahme
Titelaufnahme
- TitelAutofluorescence-based assessment of chlorine dioxide disinfection in industrial water systems / vorgelegt von Sebastian Wilfried Birr
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- Umfang1 Online-Ressource (138 Blätter) : Illustrationen, Diagramme
- HochschulschriftBergische Universität Wuppertal, Dissertation, 2025
- Verteidigung2025-06-26
- SpracheEnglisch
- DokumenttypDissertation
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Abstract
This paper presents a method for inhibiting microbial load in water-containing systems with the oxidation agent chlorine dioxide, utilising an autofluorescence-based measurement setup. The fundamental premise of this process is that oxidising agents, particularly chlorine dioxide, can damage cells, leading to death. The proteins within the cell membrane are significantly damaged, which is the reason for chlorine dioxide's extensive spectrum of action. This oxidising agent can eliminate bacteria, fungi, viruses, and even spores that are considered very resistant. This study demonstrates that bacterial fluorescence can be used to observe bacterial growth and thereby make semi-quantitative assessments of the overall bacterial count. This method measures a few hundred CFU/ml range and is suitable for various water-bearing processes. In addition, fluorescence can be used to evaluate the effectiveness of a disinfection process. This approach optimises water disinfection, whereby the required quantity can be precisely dosed and adjusted. One possible field of application could be metalworking fluids. These are highly complex solutions with many components, providing a suitable habitat for various microorganisms. Some of these components are highly reactive to chlorine dioxide, but as it turns out, some alternatives make the application possible. These are optically challenging solutions in which no measurements are feasible and can also be remedied with an optimised structure. Therefore, the setup suits clear process water or rinsing water and cooling lubricants. Based on the method developed here, a prototype will be further developed and tested during a field study, facilitating its application in industry.
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