Comprehensive characterization of deeply penetrating charged droplet signatures in the high-vacuum region of electrospray ionization mass spectrometry / Chris Vico Heintz. Wuppertal, May 15, 2025
Inhalt
- Titlepage
- Abstract
- Danksagung
- 1 Introduction
- 2 Bruker micrOTOF and Experimental Setup
- 2.1 Configuration Bruker MicrOTOF
- 2.2 Atmospheric Pressure Ionization Interfaces
- 2.3 Transfer Unit
- 2.4 Orthonogal Acceleration Stage
- 2.5 time-of-flight Analyzer
- 2.6 Detectors
- 2.6.1 Secondary Electron Multiplier Dynode (SEM)
- 2.6.2 Multi-Channel-Plate Detector (MCP)
- 2.6.3 Transient Recorder
- 2.7 Electronic Communication
- 2.8 Oscilloscope and Arduino Settings
- 2.9 Experiment Automation
- 2.10 Chemicals
- 2.11 Injection System
- 2.12 Attempting to Use a Custom HV Switch
- References
- 3 Introduction to the Cumulative Part
- 4 Observation of Droplet Signatures
- 4.1 Abstract
- 4.2 Introduction
- 4.3 Methods
- 4.4 Results and Discussion
- 4.4.1 Observation of Droplet Signatures in the Time-Resolved Ion Signal
- 4.4.2 Temporal signal width
- 4.4.3 Analysis of single mass spectra
- 4.4.4 Dependence on source and ion transfer stage parameters
- 4.5 Conclusions
- References
- 5 Long-term Experiments under Influence of Polarity Mode Switching
- 5.1 Abstract
- 5.2 Introduction
- 5.3 Methods
- 5.4 Results and discussion
- 5.4.1 Recovery of Total Ion Count as a Function of Instrument Standby Time
- 5.4.2 Signal recovery after switching the polarity mode
- 5.4.3 Fast polarity switching
- 5.4.4 Impact of droplets on signal instability
- 5.5 Conclusions
- References
- 6 Statistical Analysis of Non-Summed Mass Spectra
- 6.1 Abstract
- 6.2 Introduction
- 6.3 Methods
- 6.3.1 Instrument
- 6.3.2 Automation
- 6.3.3 Observation of Spray Stability
- 6.3.4 Chemicals
- 6.3.5 Observation of Droplet Signatures
- 6.4 Results and Discussion
- 6.4.1 Transient Spray Phenomena Induced by Polarity Switches
- 6.4.2 Raw Spectra in Dependence of ESI Source Parameters
- 6.4.3 Summed Raw Spectra
- 6.5 Conclusions
- 6.6 Supporting Materials
- References
- 7 Summary and Conclusion
- 7.1 Appearance and Recording
- 7.1.1 Waveforms in Oscillogram
- 7.1.2 Dependence of Source and Transfer Parameters
- 7.1.3 Comparison to other Ionization Techniques
- 7.2 Individual Non-Summed TOF mass spectra
- 7.2.1 Dependence of Source Parameters
- 7.2.2 Connection to ion bursts
- 7.2.3 Comparison to other Ionization Techniques
- 7.3 Long-Term Experiments
- 8 Outlook
- Declaration