Enhancing the signal interpretation and microscopical hardware concept of 3D polarized light imaging / vorgelegt von: Hendrik Wiese. Wuppertal, August 2016
Inhalt
- A short Introduction to Brain Mapping
- Basics of Birefringence Polarimetry
- Polarized Light and its Mathematical Description in Experiments
- Birefringence of Nerve Fibers
- Established Experimental Methods in 3D-PLI
- 3D-PLI Data Visualization
- Preparation of Histological Brain Slides and Volumetric Reconstruction
- Polarimetric Calibration of the LAP
- The Standard Intensity Based Calibration in 3D-PLI
- Accounting for Non-Ideal Polarization Filters
- Determination of the Filter Properties
- Discussion of the Polarimetric Calibration
- Deriving Fiber Orientations from Tiltable Specimen Stage Experiments
- A Review on Ambiguities in 3D-PLI
- Basic Theory of the Tiltable Specimen Stage Data Acquisition
- An Analytical Solution to Calculate the Inclination of a Uniaxial Indicatrix
- Deriving the Inclination from Measurement Data
- Validation for Simulated Datasets
- Validation for Experimental Datasets
- Maximum Likelihood Estimation of the Birefringence Parameters
- Noise Sensitivity of the LAP Imaging System
- Theoretical Model of the 3D-PLI Data Acquisition Process
- The Likelihood Orientation Estimation Algorithm
- Simulative Examination of the Noise Stability
- Simulation of Crossing Fibers
- Validation of the LOriE Algorithm for Experimental Datasets
- Discussion
- Conclusion
- Developing an Oblique Illumination System for 3D-PLI Microscopy
- Microscopic Techniques to Complement Planar 3D-PLI Measurements
- Basics of Geometrical Optics
- Simulating the Optical Train
- Designing an Oblique Koehler Illumination
- The Standard Koehler Illumination
- Realizing an Oblique Illumination
- Engineering and Optimization of the Optical Design
- Validating the Oblique Illumination System for 3D-PLI
- Conclusion
- Impact on Future 3D-PLI Data Interpretation Techniques
- Acknowledgments
- Mathematical Derivations
- Derivation of ne()-no(nE- no)cos2()
- The Intensity Profile for Imperfect Polarization Filters
- Proof of Bijectivity for ,trelsin(), sin()/
- Transformation between Tilting Coordinate Systems.
- The Derivation of the Phase Retardance Signal and Its Analysis
- Error Propagation of Detection Noise
- Complementary Data
- A Direct Comparison between the LOriE and the DFT-based Algorithm
- Angular Spectrum of the Diffuser
- Shape of the Condenser Lens
- Influence of the Resolution
- List of Symbols
- List of Abbreviations
- List of Figures
- List of Tables
- References