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Residues of veterinary drugs in eggs and possible explanations for their distribution between egg white and yolk / von Cornelis Adriaan Kan. 2003
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Cornelis Adriaan Kan
The work described in this thesis was carried out at the former Spelderholt Centre for Poultry Research and Extension, Beekbergen, The Netherlands between 1990 and 1996.
Professor Dr. Michael Petz, Faculty of Chemistry,
Referee: Prof. Dr. M. Petz
Co-referee: Prof. Dr. H. Guth
Acknowledgements
Parts of the work described in this thesis have already been published in:
ContentsPage
1.2 Formation of10
1.3 Residues in egg white and yolk14
1.4 Available data on drug levels in egg white and yolk15
1.5 General observations on the distribution data15
2.EXPERIMENTAL PART
2.3 Analysis of sulfonamides34
2.3.3 Solutions35
2.5 Technical results
3.RESULTS AND DISCUSSION
3.1 Introduction57
4.ANNEXES65
ABSTRACT
1.INTRODUCTION
Figure 4: Time schedule of egg formation (Gilbert 1971a)
1.2 Formation of
1.4 Available data on drug levels in egg white and yolk
1.5 General observations on the distribution data
1.8 Table 1: Literature data on drug residues in white and yolk
Compound name
Exposure way
Sulfonamides
Tetracyclines
2EXPERIMENTAL PART
2.1 Introduction
2.2 Materials and methods
2.2.1 Determination of partitioning coefficients
2.3 Analysis of sulfonamides
2.3.3 Solutions
2.4 Animal experiments
Table 2: Concentrations in the feed
Group 1: Sulfachloropyrazine
2.5 Technical results
2.5.1.1 Chromatographic conditions
Figure 13: Influence of temperature on separation of the sulfonamides (Top to bottom temperature increase from 14 till 34 oC) pH = 5, 0.01 M eluent
Figure 14: Chromatogram of blank egg white sample
Figure 15: Chromatogram of standard sulfonamide solution
2.5.1.2 Linearity of the response
2.5.1.3 Recovery percentages
RESULTS AND DISCUSSION
Introduction
Furusawa \(2001\) later stated shortly “Large
These statements nicely summarise the traditional approach to the subject of this study. Literature data and data out of our previous studies and the present one clearly indicate certain inconsistencies or mistakes in this concept.
3.4 Some points still to be tackled