Omega-3 fatty acids and oxylipins as modulators of phagocytosis in human macrophages / Rebecca Kirchhoff, formerly Rebecca Pfaff. Wuppertal, 2025
Inhalt
- Introduction and Scope
- An Optimized Ex Vivo n–3 PUFA Supplementation Strategy
- Introduction
- Experimental Section
- Chemicals and Biological Materials
- Cell Cultivation
- Supplementation Experiments
- Quantification of Oxylipin, Fatty Acyl, and Protein Levels by LC-MS/MS
- RNA Extraction and Quantitative PCR Analysis
- Statistical Analysis
- Results and Discussion
- Characterization of Key Parameters for the Ex Vivo Supplementation Strategy
- Effects of n–3 PUFA Supplementation on Macrophages FA Pattern
- Effects of n–3 PUFA Supplementation on the Oxylipin Pattern of Macrophages
- Effects of n–3 PUFA Supplementation on LPS Stimulation of M2-Like Macrophages
- Concluding Remarks
- Conclusion
- References
- Phagocytosis is differentially regulated by LPS
- Introduction
- Materials and Methods
- Chemicals and Biological Material
- Isolation and Differentiation of Primary Human Macrophages
- Phagocytosis Assay
- Quantification of Oxylipin and Protein Levels by LC-MS/MS
- Statistical Analysis
- Results
- Discussion
- Optimization of the Phagocytosis Assay
- Phagocytosis is differentially modulated by LPS in M1- and M2-like macrophages
- References
- Phagocytosis of human macrophages is elevated by n–3 PUFA
- Introduction
- Materials and Methods
- Chemicals and Biological Material
- Isolation and differentiation of primary human macrophages
- Supplementation of human macrophages with n–3 PUFA
- Phagocytosis assay
- Quantification of fatty acyl and oxylipin levels by means of LC-MS/MS
- Data analysis
- Results
- Ex vivo supplementation of primary human macrophages with n–3 PUFA
- Ex-vivo supplementation with n–3 PUFA increases phagocytosis in macrophages
- Impact of oxylipins on phagocytosis
- Discussion
- Conclusion
- References
- Concluding Remarks and Future Perspectives
- Summary
- Appendix for Chapter 2
- Appendix for Chapter 3
- Appendix for Chapter 4
- Danksagung
- Curriculum Vitae
- List of Publications