Investigation of charged droplets aspirated in the electrospray ionization process / vorgelegt von Clara Markert. Wuppertal, April 2023
Inhalt
- Abstract
- Introduction - Electrospray Ionization (ESI)
- Principles of the ESI process
- Step 1 – Droplet formation
- Step 2 - Evaporation process
- Step 3 – Ion formation and detection
- Observation of charged droplets in API MS
- Introduction
- Experimental: SCIEX Triple Quad 6500
- Observation of droplet signatures with thermometer ions as analytes
- Collision gas pressure variation
- Observation of droplet signature with reserpine as analyte
- Experimental: amaZon speed ETD
- Experimental: Bruker esquire6000
- Experimental: Agilent 6538 UHD Q-TOF
- Variation of ion source parameters
- Ion source parameters amaZon speed ETD
- Nebulizer gas pressure variation – thermometer ions
- Dry gas variation - thermometer ions
- Liquid flow - thermometer ions
- Temperature variation - thermometer ions
- Nebulizer gas pressure variation - reserpine in acetonitrile
- Nebulizer gas pressure variation – reserpine in methanol
- Liquid flow - reserpine in acetonitrile
- Ion Source Parameters SCIEX Triple Quad 6500
- Variation of transfer parameter
- Conclusion - Experiments
- Simulation of charged droplets
- Introduction
- Methods to simulate droplets
- Evaluation of critical simulation parameters
- Internal energy transfer of simulated droplets
- Droplet fragmentation
- Mobility calculations
- Conclusions - Simulations
- Outlook
- Abbreviations
- List of tables
- List of figures
- References